Been banging my head against the wall over this purity issue. I run HPLC on my peptides, thinking I've prepped everything right, but results keep coming back all over the place, sometimes way off the expected values. Tried fresh solvents and even recalibrated the gear, but nothing seems to fix it. Starting to wonder if I'm missing some basic trick or if there’s something weird happening with my samples or columns. Anyone else gone through this? Getting kind of desperate since I need reproducible numbers for my thesis this year. Would love any tips or advice you've got!